Comparative study of synovial fluid and serum protein electrophoretic pattern of river buffalo (Bubalus bubalis) in Ahvaz

Document Type : Research Paper

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Electrophoresis is a meticulous method in para-clinical science but has received less attention. In this study, the protein profile of synovial fluid of carpal joint and blood serum in buffalo was detected. Sampling was performed by arthrocentesis of 80 left and right forelimbs and obtaining blood serums of 40 Bubalus bubalis in Ahvaz industrial slaughterhouse. Samples were stratified by gender, age and left and right forelimbs. At first, the health of samples was confirmed by measuring the physical parameters of synovial fluid such as color, viscosity, transparency, and quality of mucin clot. Then, total protein was measured by Biuret method. Separating of synovial fluid protein fractions (include: albumin, alpha globulin, beta globulin, gamma globulin) was performed by electrophoresis method with cellogel kit. Statistical analysis showed that total protein, albumin, alpha globulin, beta globulin, gamma globulin in synovial fluid were significantly lower than the serum (P<0.05). The statistical analysis showed that the measured parameters in relation to age, gender and the limbs had no significant difference. Although synovial fluid, like all other biological fluid, is structurally similar to serum but is slightly different following disorders and diseases, nature and quantity of some these proteins change and analysis of the fluid can be used to identify trends of disease, prediction of the development and evaluation of the treatment processes.

Keywords


 
References
Ameri, M. & Gharib, Z. (2005) Analysis of synovial fluid from clinically healthy Iranian fat-tailed sheep. Comp. Clin. Path. 13, 4, 186-189.
 
Baniadam, A. & Razi Jalali, M. (2005) Studies on the normal synovial fluid from the carpal joints of Iranian water buffaloes: physical and biochemical parameters. Iran. J. Vet. Res. 6, 17-20.
 
Bani Ismail, Z. & Alā€Rukibat, R. (2006) Synovial fluid cell counts and total protein concentration in clinically normal fetlock joints of young dromedarian camels. J. Vet. Med. A. 53, 5, 263-265.
 
Chauhan, RS. (1995) Veterinary Clinical and Laboratory Diagnosis. Jaypee Brothers Medical, New Dehli, Vol. 1, 1th ed. PP: 20-25.
 
Decker, B., McKenzie, B.F., McGuckin, WF. & Slocumb, CH. (1959) Comparative distribution of proteins and glycoproteins of serum and synovial fluid. Arthritis. Rheum. 2, 2, 162-177.
 
Khazraneian, P., gharaghozlou, M.J., Nazifi, S., Najafi, J., Hasani Tabatabaei, M. & Youssefy, P. (2009). Microbiological and clinicopathological studies on bovine arthritis in Iran. J. Vet.Res. 61, 1, 33-38.
 
Kumar, M. & Agarwal, K. (2001) Biochemical alterations of synovial fluid in induced traumatic arthritis in buffalo calves (Bubalus bubalis). Indian. J. Vet. Surg. 22, 2, 87-91.
 
Kumar, M. & Agrawal, K. (2001) Synovial fluid changes following intra-articular synovial fluid transfusion in induced traumatic arthritis in buffalo calves (Bubalus bubalis). Indian. J. Anim. Sci. 71, 12, 1153-1155.
 
Latimer, K., Mahaffey, E. & Prasse, K. (2003) Clinical Pathology: Veterinary Laboratory Medicine. Iowa State Press, Iowa, USA. PP: 27-89.
 
Madison, J. Sommer, M. & Spencer, P. (1991) Relations among synovial membrane histopathologic findings, synovial fluid cytologic findings, and bacterial culture results in horses with suspected infectious arthritis: 64 cases (1979-1987). J. Am. Vet. Med. Assoc. 198, 9, 1655-1661.
 
Mojabi, A., Norouzian, I. & Chavoushi, M.(1991) Evaluation of some non-electrolytes in normal synovial fluid of cow. J. Vet.Res. 46, 3-4, 9-28.
 
Najizadeh, MH., Pourjafar, M., Chalmeh, AA., Badiei, K., Nazifi, S. & Naghib, M. (2014) Synovial fluid inflammatory cytokines and proteins in clinically healthy and arthritic joint of dromedary camels (Camelus dromedarius). Iran. J. Vet. Med. 8, 1, 53-57.
 
Nasim, S., Nazifi, S. & Meimandi Parizi, A. (2010) Cellular and biochemical parameters of synovial fluid of fetlock joint clinically normal Iranian fat-tailed sheep. J. Vet. Lab. Res. 1, 2, 115-124.
 
Nazifi, S., Rezakhani, A. & Gheisari, H. (1998). Physical, biochemical and cytologic properties of blood and synovial fluid in clinically normal adult camel (Camelus dromedarius). J. Vet. Med. A. 45, 1-10, 155-160.
 
Pacchiana, PD., Gilley, RS., Wallace, LJ., Hayden, DW., Feeney, DA., Jessen, CR. & Aird, B. (2004) Absolute and relative cell counts for synovial fluid from clinically normal shoulder and stifle joints in cats. J. Am. Vet. Med. Assoc. 225, 12, 1866.
 
Rohde, C., Anderson, D.E., Desrochers, A., St-jean, G., Hull, BL. & Rings, M. (2000) Synovial fluid analysis in cattle: a review of 130 cases. Vet. Surg. 29, 341- 346.
 
Sharifi Khatir, A., Qujeq, D. & Yousef Ghahhary, B. (2009) Determination of lactate level in serum and joint knee synovial fluid in patients with arthritis. Q. Horizon. Med. Sci. 15, 2, 40-45.
 
Smithies, O. (1959) Zone Electrophoresis in Starch Gels and its Application to Studies of Serum Proteins. Advances in Protein Chemistry. Vol. 14. PP: 65-113.
 
Stashak, T.S. (2002) Adams Lameness in Horses. Lippincott Williams and Wilkins, Philadelphia, 5th ed. PP: 349-357.
 
Swan, A., Amer, H. & Dieppe, P. (2002) The value of synovial fluid assays in the diagnosis of joint disease: a literature survey. Ann. Rheum. Dis. 61, 493-498.
 
Tetta, C., Camussi, G., Modena, V., Divittorio, C. & Baglioni, C. (1990) Tumor necrosis factor in serum and synovial fluid of patients with active and severe rheumatoid arthritis. Ann. Rheum. Dis, 49, 665-667.
 
Van Pelt, R. (1974) Interpretation of synovial fluid findings in the horse. J. Am. Vet. Med. Assoc. 165, 1, 91-95.
 
Vishal, A.A., Mishra, A. & Raychaudhuri, SR. (2011). A Double Blind, Randomized, Placebo Controlled Clinical Study Evaluates the Early Efficacy of Aflapin® in Subjects with Osteoarthritis of Knee. Int. J. Med. Sci. 8, 7, 615-622.
 
Watkins, G. H. (2000). Arthritis, In: Martin, W. B. and Aitken, I. D., Diseases of Sheep, Blackwell, Oxford, 3th ed. PP: 249–253.